The church of Margao was founded by the Jesuits in 1564. The first mass was celebrated on Saturday in Albis (i.e. on 11th April, the Saturday before the second Sunday of Easter) of 1565.
The first church building was constructed at the initiative of the then Provincial of the Jesuits, Fr. Antonio Quadros, the choice of the site having been made by the first Archbishop of Goa, D. Gaspar Leao Pereira (1560-67). This church was destroyed by fire during the invasion by the Muslims in 1571. The second, built at the same spot and of the same material was destroyed in 1579, eight years later. The third edition had a very short duration.
The fourth church building was built a little further away from the first, in 1589 and consecrated in 1595; it was extended in 1604. This whole edifice was demolished in 1645 and 30 years later in 1675, the present church building, the fifth one, was built. It's frontispiece is majestic and artistic and at it's center one can see, high and above, the Holy Spirit and below it the Cenacle.